The Cytoplasmic and Periplasmic Expression Levels and Folding of Organophosphorus Hydrolase Enzyme in Escherichia coli
نویسندگان
چکیده
منابع مشابه
the cytoplasmic and periplasmic expression levels and folding of organophosphorus hydrolase enzyme in escherichia coli
conclusions the activities studied by the production of pnp were determined by following the increase at 410 nm. the maximum pnp was produced at 30°c with an optical density of 10.62 in the presence of cytoplasmic expression of oph (pet21a-opd). consequently, our results suggest cytoplasmic expression system as an appropriate candidate with a high amount of oph in spite of inclusion body format...
متن کاملThe Cytoplasmic and Periplasmic Expression Levels and Folding of Organophosphorus Hydrolase Enzyme in Escherichia coli.
BACKGROUND Organophosphorus hydrolase (OPH) is a type of organophosphate-degrading enzyme which is widely used in the bioremediation process. OBJECTIVES In this study, the periplasmic and cytoplasmic productions and the activity of recombinant OPH in Escherichia coli were investigated and compared using two pET systems (pET21a and pET26b). MATERIALS AND METHODS The sequence encoding the opd...
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The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2015
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.17790